Comparative Genomics: Differential Display and Subtractive Hybridization
نویسندگان
چکیده
منابع مشابه
The hepatic transcriptome in human liver disease
The transcriptome is the mRNA transcript pool in a cell, organ or tissue with the liver transcriptome being amongst the most complex of any organ. Functional genomics methodologies are now being widely utilized to study transcriptomes including the hepatic transcriptome. This review outlines commonly used methods of transcriptome analysis, especially gene array analysis, focusing on publication...
متن کاملSuppression Subtractive Hybridization in Penaeid Prawns: An Approach in Identifying Diseases and Differentially Expressed Genes
Alteration in gene transcripts is related to a wide spectrum of physiological and pathological changes in cells. Therefore, the identification of differential gene expression can be used to understand biological processes at a molecular level [1-4]. Various techniques such as Expressed Sequence Tags (EST), differential display, Serial Analysis of Gene Expression (SAGE), cDNA microarray, and sub...
متن کاملA Mathematical Model for Suppression Subtractive Hybridization
Suppression subtractive hybridization (SSH) is frequently used to unearth differentially expressed genes on a whole-genome scale. Its versatility is based on combining cDNA library subtraction and normalization, which allows the isolation of sequences of varying degrees of abundance and differential expression. SSH is a complex process with many adjustable parameters that affect the outcome of ...
متن کاملDifferential gene expression analysis in fish exposed to endocrine disrupting compounds.
This review discusses various methodologies that can be used to understand, at the gene level, the consequences to fish upon exposure to endocrine disrupting compounds (EDCs). Several approaches for measuring expression of gene transcripts are discussed, including directed approaches, such as Northern blotting and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) as well as ...
متن کاملRegion-specific transcriptional activity in the genome of Streptomyces coelicolor A3(2).
Transcriptional analysis of microbial genomes is an important component of functional genomics. Strategies such as hybridization of labeled total RNA against ordered clone libraries or differential-display approaches have already been carried out to identify expressed genes. We describe here an additional method which applies subtractive hybridization between genome-specific DNA and total RNA f...
متن کامل